DESCRIPTION: Type IX collagen is purified from pepsin-solubilized articular porcine cartilage by repeated salt precipitation. Type IX collagen is one of three types of collagen which makes up cartilage fibrils. Type IX collagen consists of three genetically distinct polypeptides which are cross-linked by disulfide bonds and is digested into high molecular weight (HMW) fragments and low molecular weight (LMW) fragments by pepsin-digestion for solubilization from tissues. The ratio of HMW and LMW varies
between individual batches.
APPLICATION: Use as an immunizing antigen to generate antibodies, as an ELISA antigen to detect anti-type IX collagen antibodies, and as a standard for gel analysis.
NOTE: Anti-type IX collagen antibodies which cross-react to autologous type IX collagen may induce
polychondritis in certain strains of mice.
QUANTITY: 5 mg
FORM: Lyophilized powder
SOURCE: Porcine
MOLECULAR WEIGHT: Intact type IX collagen: approximately 220 kDa, by 8% gel analysis under non-reduced conditions,pepsin-solubilized type IX collagen is a mixture of three HMW (150, 135, and 120 kDa) and one LMW(35 kDa) fragments.
PURITY: >90% by SDS-PAGE
SOLUBILITY: Type IX collagen can be dissolved at 4 mg/ml in acidic solution, such as 0.01-0.05M acetic acid (pH3.0-3.3) or 0.15M citrate buffer (pH 3.6), by stirring at 4°C overnight. To neutralize the solution, add 1/10 volume of a 10X concentrated neutral buffer containing 1.5M NaCl or dialyze the solution against a neutral buffer containing 0.15M NaCl.
STORAGE TEMPERATURE: 4°C in the dark. After reconstitution, store at –20°C. Collagen may degrade under neutral conditions.
STABILITY: 2 years
REFERENCES: 1. Shimokomaki M, Duance VC, Bailey AJ. FEBS Lett. 121:51-54 (1980)
2. Reese CA, Wiedemann H, Kuhn K, Mayne R. Biochemistry: 21:826-830 (1982)
3. vander Rest M, Mayne R, Ninomiya Y, et al. J Biol Chem. 260:220-225 (1985)